ABSTRACT
Background: Colon cancer is one of the most common malignant tumor of digestive system. There are differences in pathogenesis, biological behavior, gene expression between left and right hemicolon cancer. Aims: To investigate the differences in clinicopathological features, microsatellite instability (MSI) and K-ras gene mutation between left and right hemicolon cancer. Methods: Data of 144 patients with colon cancer diagnosed by postoperative pathology from June 2017 to June 2018 at Qingdao Municipal Hospital were collected. MSI was assessed by immunohistochemistry, K-ras gene mutation was detected by PCR. The differences in clinicopathological features, MSI and K-ras gene mutation between the two groups were compared. Results: Right hemicolon cancer was more common in female, and left hemicolon cancer was more common in male. The incidence of lymph node metastasis, positivity rate of CEA and MSI in right hemicolon cancer were significantly higher than left hemicolon cancer (P<0.01), while the K-ras gene mutation rate in left hemicolon cancer was significantly higher than right hemicolon cancer (P<0.05). The K-ras gene mutation in left hemicolon cancer was correlated with gender, lymph node metastasis and positivity rate of CEA (P<0.05). MSI in right hemicolon cancer was correlated with gender, age, and lymph node metastasis (P<0.05). Conclusions: There are differences in the MSI and K-ras gene mutation between left hemicolon cancer and right hemicolon cancer, which can be used as the reference for diagnosis, individualized treatment and prognosis of colon cancer.
ABSTRACT
Objective@#To investigate the dynamic change of paraquant-induced kidney injury in rats and the protective effect of edaravone.@*Methods@#Eighty SD rats were randomly divided into 4 groups: the normal control group, paraquat poisoning group, edaravone treatment group and edaravone control group. The normal control group of 8 rats were given 1 ml of 0.9% sodium chloride through the abdominal cavity, and the same amount of fluid into the abdominal cavity after 30 minutes. The paraquat poisoning group of 24 rats were given 1 ml of paraquat solution (20 mg/kg) through the abdominal cavity to build poisoning models, and the same amount of 0.9% sodium chloride was injected into the abdominal cavity after 30 minutes. The edaravone treatment group of 24 rats were given edaravone (5 mg/kg) through the abdominal cavity after 30 minutes when the poisoning models were set up. The edaravone control group of 24 rats were given 1 ml of 0.9% sodium chloride through the abdominal cavity, and edaravone (5 mg/kg) was injected into the abdominal cavity after 30 minutes. In addition to the normal control group, the other groups processed 1 times a day to mantain 7 d. On 1, 3, 7, 21 d several rats in each group were excuted and the kidney tissue and serum samples were collected, then each pathological changes of the kidney were observed with light microscopy. Serum creatinine, KIM-1, NGAL were measured by ELISA, the expression of HSP70 protein in kidney were observed with immunohistochemical staining.@*Results@#The pathological examination reveald that the damage of kidney tissue in the paraquat group was the most serious on 3 d, and the damage was consistently alleviated in edaravone treatment group at the same time, renal fibrosisn was unseen in each group until 21 d. Compared with normal control group, there was no statistically significant in edaravone control group (P>0.05) . The KIM-1 in blood and kidney in paraquat poisoning group were markedly increased in 1 d (P<0.05) . The NGAL in blood and creatinine were markedly increased in d7 (P<0.05) . The NGAL in kidney increased over time, but had no statistically difference with the control group (P>0.05) .Compared with paraquat poisoning group, the serum creatinine, KIM-1 in blood and kidney, the KIM-1 in kidney had decreased significantly in edaravone treatment group (P<0.05) . The NGAL in kidney has no statistically significant compared with the poisoning group (P>0.05) . HSP70 expression of kidney tissue in edaravone treatment group had significantly increased in d3 compared with the paraquat poisoning group (P<0.05) .@*Conclusion@#Edaravone can prompt a significant rise of HSP70 in kidney tissue, reduce KIM-1 and NGAL levels, and play a protective role in kidney injury of acute paraquat poisoning.